Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry

Research output: Contribution to journalJournal articlepeer-review

Standard

Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry. / Haselmann, K F; Budnik, B A; Olsen, Jesper Velgaard; Nielsen, Michael Lund; Reis, C A; Clausen, H; Johnsen, A H; Zubarev, R A.

In: Analytical Chemistry, Vol. 73, No. 13, 2001, p. 2998-3005.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Haselmann, KF, Budnik, BA, Olsen, JV, Nielsen, ML, Reis, CA, Clausen, H, Johnsen, AH & Zubarev, RA 2001, 'Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry', Analytical Chemistry, vol. 73, no. 13, pp. 2998-3005.

APA

Haselmann, K. F., Budnik, B. A., Olsen, J. V., Nielsen, M. L., Reis, C. A., Clausen, H., Johnsen, A. H., & Zubarev, R. A. (2001). Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry. Analytical Chemistry, 73(13), 2998-3005.

Vancouver

Haselmann KF, Budnik BA, Olsen JV, Nielsen ML, Reis CA, Clausen H et al. Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry. Analytical Chemistry. 2001;73(13):2998-3005.

Author

Haselmann, K F ; Budnik, B A ; Olsen, Jesper Velgaard ; Nielsen, Michael Lund ; Reis, C A ; Clausen, H ; Johnsen, A H ; Zubarev, R A. / Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry. In: Analytical Chemistry. 2001 ; Vol. 73, No. 13. pp. 2998-3005.

Bibtex

@article{564a11c0457111df928f000ea68e967b,
title = "Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry",
abstract = "A combination of external accumulation (XA) with electron capture dissociation (ECD) improves the electron capture efficiency, shortens the analysis time, and allows for rapid integration of multiple scans in Fourier transform mass spectrometry. This improves the signal-to-noise ratio and increases the number of detected products, including structurally important MS3 fragments. With XA-ECD, the range of the labile species amenable to ECD is significantly extended. Examples include the first-time determination of the positions of six GalNAc groups in a 60-residue peptide, five sialic acid and six O-linked GalNAc groups in a 25-residue peptide, and the sulfate group position in a 11-residue peptide. Even weakly bound supramolecular aggregates, including nonspecific peptide complexes, can be analyzed with XA-ECD. Preliminary results are reported on high-rate XA-ECD that uses an indirectly heated dispenser cathode as an electron source. This shortens the irradiation time to > or = 1 ms and increases the acquisition rate to 3 scans/s, an improvement by a factor of 10-100.",
author = "Haselmann, {K F} and Budnik, {B A} and Olsen, {Jesper Velgaard} and Nielsen, {Michael Lund} and Reis, {C A} and H Clausen and Johnsen, {A H} and Zubarev, {R A}",
note = "Keywords: Adrenocorticotropic Hormone; Amino Acid Sequence; Bombesin; Fourier Analysis; Glycosylation; Humans; Mass Spectrometry; Melitten; Molecular Sequence Data; Mucin-1; Peptide Fragments",
year = "2001",
language = "English",
volume = "73",
pages = "2998--3005",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "13",

}

RIS

TY - JOUR

T1 - Advantages of external accumulation for electron capture dissociation in Fourier transform mass spectrometry

AU - Haselmann, K F

AU - Budnik, B A

AU - Olsen, Jesper Velgaard

AU - Nielsen, Michael Lund

AU - Reis, C A

AU - Clausen, H

AU - Johnsen, A H

AU - Zubarev, R A

N1 - Keywords: Adrenocorticotropic Hormone; Amino Acid Sequence; Bombesin; Fourier Analysis; Glycosylation; Humans; Mass Spectrometry; Melitten; Molecular Sequence Data; Mucin-1; Peptide Fragments

PY - 2001

Y1 - 2001

N2 - A combination of external accumulation (XA) with electron capture dissociation (ECD) improves the electron capture efficiency, shortens the analysis time, and allows for rapid integration of multiple scans in Fourier transform mass spectrometry. This improves the signal-to-noise ratio and increases the number of detected products, including structurally important MS3 fragments. With XA-ECD, the range of the labile species amenable to ECD is significantly extended. Examples include the first-time determination of the positions of six GalNAc groups in a 60-residue peptide, five sialic acid and six O-linked GalNAc groups in a 25-residue peptide, and the sulfate group position in a 11-residue peptide. Even weakly bound supramolecular aggregates, including nonspecific peptide complexes, can be analyzed with XA-ECD. Preliminary results are reported on high-rate XA-ECD that uses an indirectly heated dispenser cathode as an electron source. This shortens the irradiation time to > or = 1 ms and increases the acquisition rate to 3 scans/s, an improvement by a factor of 10-100.

AB - A combination of external accumulation (XA) with electron capture dissociation (ECD) improves the electron capture efficiency, shortens the analysis time, and allows for rapid integration of multiple scans in Fourier transform mass spectrometry. This improves the signal-to-noise ratio and increases the number of detected products, including structurally important MS3 fragments. With XA-ECD, the range of the labile species amenable to ECD is significantly extended. Examples include the first-time determination of the positions of six GalNAc groups in a 60-residue peptide, five sialic acid and six O-linked GalNAc groups in a 25-residue peptide, and the sulfate group position in a 11-residue peptide. Even weakly bound supramolecular aggregates, including nonspecific peptide complexes, can be analyzed with XA-ECD. Preliminary results are reported on high-rate XA-ECD that uses an indirectly heated dispenser cathode as an electron source. This shortens the irradiation time to > or = 1 ms and increases the acquisition rate to 3 scans/s, an improvement by a factor of 10-100.

M3 - Journal article

C2 - 11467546

VL - 73

SP - 2998

EP - 3005

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 13

ER -

ID: 19160427