Cell-Type- and Brain-Region-Resolved Mouse Brain Lipidome

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Documents

  • Dirk Fitzner
  • Jakob M Bader
  • Horst Penkert
  • Caroline G Bergner
  • Minhui Su
  • Marie-Theres Weil
  • Michal A Surma
  • Mann, Matthias
  • Christian Klose
  • Mikael Simons

Gene and protein expression data provide useful resources for understanding brain function, but little is known about the lipid composition of the brain. Here, we perform quantitative shotgun lipidomics, which enables a cell-type-resolved assessment of the mouse brain lipid composition. We quantify around 700 lipid species and evaluate lipid features including fatty acyl chain length, hydroxylation, and number of acyl chain double bonds, thereby identifying cell-type- and brain-region-specific lipid profiles in adult mice, as well as in aged mice, in apolipoprotein-E-deficient mice, in a model of Alzheimer's disease, and in mice fed different diets. We also integrate lipid with protein expression profiles to predict lipid pathways enriched in specific cell types, such as fatty acid β-oxidation in astrocytes and sphingolipid metabolism in microglia. This resource complements existing brain atlases of gene and protein expression and may be useful for understanding the role of lipids in brain function.

Original languageEnglish
Article number108132
JournalCell Reports
Volume32
Number of pages21
ISSN2211-1247
DOIs
Publication statusPublished - 2020

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