Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars
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Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars. / Groff, Dan; Benke, Peter I; Batth, Tanveer S; Bokinsky, Gregory; Petzold, Christopher J; Adams, Paul D; Keasling, Jay D.
In: Applied and Environmental Microbiology, Vol. 78, No. 7, 04.2012, p. 2221-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars
AU - Groff, Dan
AU - Benke, Peter I
AU - Batth, Tanveer S
AU - Bokinsky, Gregory
AU - Petzold, Christopher J
AU - Adams, Paul D
AU - Keasling, Jay D
PY - 2012/4
Y1 - 2012/4
N2 - Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.
AB - Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.
KW - Arabinose
KW - Biofuels
KW - Biotechnology
KW - Culture Media
KW - Escherichia coli
KW - Escherichia coli Proteins
KW - Ethanol
KW - Fermentation
KW - Gene Expression Regulation, Bacterial
KW - Gene Expression Regulation, Enzymologic
KW - Polysaccharides
KW - Transcription Factors
KW - Xylose
U2 - 10.1128/AEM.06761-11
DO - 10.1128/AEM.06761-11
M3 - Journal article
C2 - 22286982
VL - 78
SP - 2221
EP - 2229
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 7
ER -
ID: 68164273