Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars. / Groff, Dan; Benke, Peter I; Batth, Tanveer S; Bokinsky, Gregory; Petzold, Christopher J; Adams, Paul D; Keasling, Jay D.

In: Applied and Environmental Microbiology, Vol. 78, No. 7, 04.2012, p. 2221-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Groff, D, Benke, PI, Batth, TS, Bokinsky, G, Petzold, CJ, Adams, PD & Keasling, JD 2012, 'Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars', Applied and Environmental Microbiology, vol. 78, no. 7, pp. 2221-9. https://doi.org/10.1128/AEM.06761-11

APA

Groff, D., Benke, P. I., Batth, T. S., Bokinsky, G., Petzold, C. J., Adams, P. D., & Keasling, J. D. (2012). Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars. Applied and Environmental Microbiology, 78(7), 2221-9. https://doi.org/10.1128/AEM.06761-11

Vancouver

Groff D, Benke PI, Batth TS, Bokinsky G, Petzold CJ, Adams PD et al. Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars. Applied and Environmental Microbiology. 2012 Apr;78(7):2221-9. https://doi.org/10.1128/AEM.06761-11

Author

Groff, Dan ; Benke, Peter I ; Batth, Tanveer S ; Bokinsky, Gregory ; Petzold, Christopher J ; Adams, Paul D ; Keasling, Jay D. / Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars. In: Applied and Environmental Microbiology. 2012 ; Vol. 78, No. 7. pp. 2221-9.

Bibtex

@article{86b203c183a342b58a9de70459a1c77c,
title = "Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars",
abstract = "Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.",
keywords = "Arabinose, Biofuels, Biotechnology, Culture Media, Escherichia coli, Escherichia coli Proteins, Ethanol, Fermentation, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Polysaccharides, Transcription Factors, Xylose",
author = "Dan Groff and Benke, {Peter I} and Batth, {Tanveer S} and Gregory Bokinsky and Petzold, {Christopher J} and Adams, {Paul D} and Keasling, {Jay D}",
year = "2012",
month = apr,
doi = "10.1128/AEM.06761-11",
language = "English",
volume = "78",
pages = "2221--9",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars

AU - Groff, Dan

AU - Benke, Peter I

AU - Batth, Tanveer S

AU - Bokinsky, Gregory

AU - Petzold, Christopher J

AU - Adams, Paul D

AU - Keasling, Jay D

PY - 2012/4

Y1 - 2012/4

N2 - Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.

AB - Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.

KW - Arabinose

KW - Biofuels

KW - Biotechnology

KW - Culture Media

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Ethanol

KW - Fermentation

KW - Gene Expression Regulation, Bacterial

KW - Gene Expression Regulation, Enzymologic

KW - Polysaccharides

KW - Transcription Factors

KW - Xylose

U2 - 10.1128/AEM.06761-11

DO - 10.1128/AEM.06761-11

M3 - Journal article

C2 - 22286982

VL - 78

SP - 2221

EP - 2229

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

ER -

ID: 68164273