Single muscle fiber proteomics reveals unexpected mitochondrial specialization

Research output: Contribution to journalJournal articleResearchpeer-review

Marta Murgia, Nagarjuna Nagaraj, Atul S Deshmukh, Marlis Zeiler, Pasqua Cancellara, Irene Moretti, Carlo Reggiani, Stefano Schiaffino, Matthias Mann

Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

Original languageEnglish
JournalE M B O Reports
Volume16
Issue number3
Pages (from-to)387-95
Number of pages9
ISSN1469-221X
DOIs
Publication statusPublished - 1 Mar 2015

ID: 139977849