Single muscle fiber proteomics reveals unexpected mitochondrial specialization

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Single muscle fiber proteomics reveals unexpected mitochondrial specialization. / Murgia, Marta; Nagaraj, Nagarjuna; Deshmukh, Atul S; Zeiler, Marlis; Cancellara, Pasqua; Moretti, Irene; Reggiani, Carlo; Schiaffino, Stefano; Mann, Matthias.

In: E M B O Reports, Vol. 16, No. 3, 01.03.2015, p. 387-95.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Murgia, M, Nagaraj, N, Deshmukh, AS, Zeiler, M, Cancellara, P, Moretti, I, Reggiani, C, Schiaffino, S & Mann, M 2015, 'Single muscle fiber proteomics reveals unexpected mitochondrial specialization', E M B O Reports, vol. 16, no. 3, pp. 387-95. https://doi.org/10.15252/embr.201439757

APA

Murgia, M., Nagaraj, N., Deshmukh, A. S., Zeiler, M., Cancellara, P., Moretti, I., Reggiani, C., Schiaffino, S., & Mann, M. (2015). Single muscle fiber proteomics reveals unexpected mitochondrial specialization. E M B O Reports, 16(3), 387-95. https://doi.org/10.15252/embr.201439757

Vancouver

Murgia M, Nagaraj N, Deshmukh AS, Zeiler M, Cancellara P, Moretti I et al. Single muscle fiber proteomics reveals unexpected mitochondrial specialization. E M B O Reports. 2015 Mar 1;16(3):387-95. https://doi.org/10.15252/embr.201439757

Author

Murgia, Marta ; Nagaraj, Nagarjuna ; Deshmukh, Atul S ; Zeiler, Marlis ; Cancellara, Pasqua ; Moretti, Irene ; Reggiani, Carlo ; Schiaffino, Stefano ; Mann, Matthias. / Single muscle fiber proteomics reveals unexpected mitochondrial specialization. In: E M B O Reports. 2015 ; Vol. 16, No. 3. pp. 387-95.

Bibtex

@article{f80c057993a84c29838b1595639ad06e,
title = "Single muscle fiber proteomics reveals unexpected mitochondrial specialization",
abstract = "Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.",
author = "Marta Murgia and Nagarjuna Nagaraj and Deshmukh, {Atul S} and Marlis Zeiler and Pasqua Cancellara and Irene Moretti and Carlo Reggiani and Stefano Schiaffino and Matthias Mann",
note = "{\textcopyright} 2015 The Authors. Published under the terms of the CC BY NC ND 4.0 license.",
year = "2015",
month = mar,
day = "1",
doi = "10.15252/embr.201439757",
language = "English",
volume = "16",
pages = "387--95",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Single muscle fiber proteomics reveals unexpected mitochondrial specialization

AU - Murgia, Marta

AU - Nagaraj, Nagarjuna

AU - Deshmukh, Atul S

AU - Zeiler, Marlis

AU - Cancellara, Pasqua

AU - Moretti, Irene

AU - Reggiani, Carlo

AU - Schiaffino, Stefano

AU - Mann, Matthias

N1 - © 2015 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

AB - Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

U2 - 10.15252/embr.201439757

DO - 10.15252/embr.201439757

M3 - Journal article

C2 - 25643707

VL - 16

SP - 387

EP - 395

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 3

ER -

ID: 139977849