Recycling of modified H2A-H2B provides short-term memory of chromatin states
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Recycling of modified H2A-H2B provides short-term memory of chromatin states. / Flury, Valentin; Reverón-Gómez, Nazaret; Alcaraz, Nicolas; Stewart-Morgan, Kathleen R.; Wenger, Alice; Klose, Robert J.; Groth, Anja.
In: Cell, Vol. 186, No. 5, 2023, p. 1050-1065.e19.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Recycling of modified H2A-H2B provides short-term memory of chromatin states
AU - Flury, Valentin
AU - Reverón-Gómez, Nazaret
AU - Alcaraz, Nicolas
AU - Stewart-Morgan, Kathleen R.
AU - Wenger, Alice
AU - Klose, Robert J.
AU - Groth, Anja
N1 - Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states.
AB - Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states.
U2 - 10.1016/j.cell.2023.01.007
DO - 10.1016/j.cell.2023.01.007
M3 - Journal article
C2 - 36750094
VL - 186
SP - 1050-1065.e19
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -
ID: 337355397