Origin of replication in circular prokaryotic chromosomes
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Origin of replication in circular prokaryotic chromosomes. / Worning, Peder; Jensen, Lars J; Hallin, Peter Fischer; Staerfeldt, Hans-Henrik; Ussery, David.
In: Environmental Microbiology, Vol. 8, No. 2, 2006, p. 353-61.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Origin of replication in circular prokaryotic chromosomes
AU - Worning, Peder
AU - Jensen, Lars J
AU - Hallin, Peter Fischer
AU - Staerfeldt, Hans-Henrik
AU - Ussery, David
PY - 2006
Y1 - 2006
N2 - To predict origins of replication in prokaryotic chromosomes, we analyse the leading and lagging strands of 200 chromosomes for differences in oligomer composition and show that these correlate strongly with taxonomic grouping, lifestyle and molecular details of the replication process. While all bacteria have a preference for Gs over Cs on the leading strand, we discover that the direction of the A/T skew is determined by the polymerase-alpha subunit that replicates the leading strand. The strength of the strand bias varies greatly between both phyla and environments and appears to correlate with growth rate. Finally we observe much greater diversity of skew among archaea than among bacteria. We have developed a program that accurately locates the origins of replication by measuring the differences between leading and lagging strand of all oligonucleotides up to 8 bp in length. The program and results for all publicly available genomes are available from https://www.cbs.dtu.dk/services/GenomeAtlas/suppl/origin.
AB - To predict origins of replication in prokaryotic chromosomes, we analyse the leading and lagging strands of 200 chromosomes for differences in oligomer composition and show that these correlate strongly with taxonomic grouping, lifestyle and molecular details of the replication process. While all bacteria have a preference for Gs over Cs on the leading strand, we discover that the direction of the A/T skew is determined by the polymerase-alpha subunit that replicates the leading strand. The strength of the strand bias varies greatly between both phyla and environments and appears to correlate with growth rate. Finally we observe much greater diversity of skew among archaea than among bacteria. We have developed a program that accurately locates the origins of replication by measuring the differences between leading and lagging strand of all oligonucleotides up to 8 bp in length. The program and results for all publicly available genomes are available from https://www.cbs.dtu.dk/services/GenomeAtlas/suppl/origin.
U2 - 10.1111/j.1462-2920.2005.00917.x
DO - 10.1111/j.1462-2920.2005.00917.x
M3 - Journal article
C2 - 16423021
VL - 8
SP - 353
EP - 361
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 2
ER -
ID: 40749297