Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. / Alonso-Gutierrez, Jorge; Chan, Rossana; Batth, Tanveer S; Adams, Paul D; Keasling, Jay D; Petzold, Christopher J; Lee, Taek Soon.

In: Metabolic Engineering, Vol. 19, 09.2013, p. 33-41.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Alonso-Gutierrez, J, Chan, R, Batth, TS, Adams, PD, Keasling, JD, Petzold, CJ & Lee, TS 2013, 'Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production', Metabolic Engineering, vol. 19, pp. 33-41. https://doi.org/10.1016/j.ymben.2013.05.004

APA

Alonso-Gutierrez, J., Chan, R., Batth, T. S., Adams, P. D., Keasling, J. D., Petzold, C. J., & Lee, T. S. (2013). Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metabolic Engineering, 19, 33-41. https://doi.org/10.1016/j.ymben.2013.05.004

Vancouver

Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ et al. Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metabolic Engineering. 2013 Sep;19:33-41. https://doi.org/10.1016/j.ymben.2013.05.004

Author

Alonso-Gutierrez, Jorge ; Chan, Rossana ; Batth, Tanveer S ; Adams, Paul D ; Keasling, Jay D ; Petzold, Christopher J ; Lee, Taek Soon. / Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. In: Metabolic Engineering. 2013 ; Vol. 19. pp. 33-41.

Bibtex

@article{04cf82626ab841f685aa5a44bb1cc3c2,
title = "Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production",
abstract = "Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative.",
author = "Jorge Alonso-Gutierrez and Rossana Chan and Batth, {Tanveer S} and Adams, {Paul D} and Keasling, {Jay D} and Petzold, {Christopher J} and Lee, {Taek Soon}",
note = "{\textcopyright} 2013 Elsevier Inc. All rights reserved.",
year = "2013",
month = sep,
doi = "10.1016/j.ymben.2013.05.004",
language = "English",
volume = "19",
pages = "33--41",
journal = "Metabolic Engineering",
issn = "1096-7176",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

AU - Alonso-Gutierrez, Jorge

AU - Chan, Rossana

AU - Batth, Tanveer S

AU - Adams, Paul D

AU - Keasling, Jay D

AU - Petzold, Christopher J

AU - Lee, Taek Soon

N1 - © 2013 Elsevier Inc. All rights reserved.

PY - 2013/9

Y1 - 2013/9

N2 - Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative.

AB - Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative.

U2 - 10.1016/j.ymben.2013.05.004

DO - 10.1016/j.ymben.2013.05.004

M3 - Journal article

C2 - 23727191

VL - 19

SP - 33

EP - 41

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

ER -

ID: 68162550