Enhancing fatty acid production by the expression of the regulatory transcription factor FadR

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Enhancing fatty acid production by the expression of the regulatory transcription factor FadR. / Zhang, Fuzhong; Ouellet, Mario; Batth, Tanveer S; Adams, Paul D; Petzold, Christopher J; Mukhopadhyay, Aindrila; Keasling, Jay D.

In: Metabolic Engineering, Vol. 14, No. 6, 11.2012, p. 653-60.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, F, Ouellet, M, Batth, TS, Adams, PD, Petzold, CJ, Mukhopadhyay, A & Keasling, JD 2012, 'Enhancing fatty acid production by the expression of the regulatory transcription factor FadR', Metabolic Engineering, vol. 14, no. 6, pp. 653-60. https://doi.org/10.1016/j.ymben.2012.08.009

APA

Zhang, F., Ouellet, M., Batth, T. S., Adams, P. D., Petzold, C. J., Mukhopadhyay, A., & Keasling, J. D. (2012). Enhancing fatty acid production by the expression of the regulatory transcription factor FadR. Metabolic Engineering, 14(6), 653-60. https://doi.org/10.1016/j.ymben.2012.08.009

Vancouver

Zhang F, Ouellet M, Batth TS, Adams PD, Petzold CJ, Mukhopadhyay A et al. Enhancing fatty acid production by the expression of the regulatory transcription factor FadR. Metabolic Engineering. 2012 Nov;14(6):653-60. https://doi.org/10.1016/j.ymben.2012.08.009

Author

Zhang, Fuzhong ; Ouellet, Mario ; Batth, Tanveer S ; Adams, Paul D ; Petzold, Christopher J ; Mukhopadhyay, Aindrila ; Keasling, Jay D. / Enhancing fatty acid production by the expression of the regulatory transcription factor FadR. In: Metabolic Engineering. 2012 ; Vol. 14, No. 6. pp. 653-60.

Bibtex

@article{b58631d67229431982c642e44deeec10,
title = "Enhancing fatty acid production by the expression of the regulatory transcription factor FadR",
abstract = "Fatty acids are important precursors to biofuels. The Escherichia coli FadR is a transcription factor that regulates several processes in fatty acid biosynthesis, degradation, and membrane transport. By tuning the expression of FadR in an engineered E. coli host, we were able to increase fatty acid titer by 7.5-fold over our previously engineered fatty acid-producing strain, reaching 5.2±0.5g/L and 73% of the theoretical yield. The mechanism by which FadR enhanced fatty acid yield was studied by whole-genome transcriptional analysis (microarray) and targeted proteomics. Overexpression of FadR led to transcriptional changes for many genes, including genes involved in fatty acid pathways. The biggest transcriptional changes in fatty acid pathway genes included fabB, fabF, and accA. Overexpression of any of these genes alone did not result in a high yield comparable to fadR expression, indicating that FadR enhanced fatty acid production globally by tuning the expression levels of many genes to optimal levels.",
keywords = "3-Oxoacyl-(Acyl-Carrier-Protein) Synthase, Acetyl-CoA Carboxylase, Acetyltransferases, Bacterial Proteins, Escherichia coli, Escherichia coli Proteins, Fatty Acid Synthetase Complex, Type II, Fatty Acids, Gene Expression Regulation, Enzymologic, Genetic Enhancement, Regulatory Elements, Transcriptional, Repressor Proteins",
author = "Fuzhong Zhang and Mario Ouellet and Batth, {Tanveer S} and Adams, {Paul D} and Petzold, {Christopher J} and Aindrila Mukhopadhyay and Keasling, {Jay D}",
note = "Copyright {\textcopyright} 2012. Published by Elsevier Inc.",
year = "2012",
month = nov,
doi = "10.1016/j.ymben.2012.08.009",
language = "English",
volume = "14",
pages = "653--60",
journal = "Metabolic Engineering",
issn = "1096-7176",
publisher = "Academic Press",
number = "6",

}

RIS

TY - JOUR

T1 - Enhancing fatty acid production by the expression of the regulatory transcription factor FadR

AU - Zhang, Fuzhong

AU - Ouellet, Mario

AU - Batth, Tanveer S

AU - Adams, Paul D

AU - Petzold, Christopher J

AU - Mukhopadhyay, Aindrila

AU - Keasling, Jay D

N1 - Copyright © 2012. Published by Elsevier Inc.

PY - 2012/11

Y1 - 2012/11

N2 - Fatty acids are important precursors to biofuels. The Escherichia coli FadR is a transcription factor that regulates several processes in fatty acid biosynthesis, degradation, and membrane transport. By tuning the expression of FadR in an engineered E. coli host, we were able to increase fatty acid titer by 7.5-fold over our previously engineered fatty acid-producing strain, reaching 5.2±0.5g/L and 73% of the theoretical yield. The mechanism by which FadR enhanced fatty acid yield was studied by whole-genome transcriptional analysis (microarray) and targeted proteomics. Overexpression of FadR led to transcriptional changes for many genes, including genes involved in fatty acid pathways. The biggest transcriptional changes in fatty acid pathway genes included fabB, fabF, and accA. Overexpression of any of these genes alone did not result in a high yield comparable to fadR expression, indicating that FadR enhanced fatty acid production globally by tuning the expression levels of many genes to optimal levels.

AB - Fatty acids are important precursors to biofuels. The Escherichia coli FadR is a transcription factor that regulates several processes in fatty acid biosynthesis, degradation, and membrane transport. By tuning the expression of FadR in an engineered E. coli host, we were able to increase fatty acid titer by 7.5-fold over our previously engineered fatty acid-producing strain, reaching 5.2±0.5g/L and 73% of the theoretical yield. The mechanism by which FadR enhanced fatty acid yield was studied by whole-genome transcriptional analysis (microarray) and targeted proteomics. Overexpression of FadR led to transcriptional changes for many genes, including genes involved in fatty acid pathways. The biggest transcriptional changes in fatty acid pathway genes included fabB, fabF, and accA. Overexpression of any of these genes alone did not result in a high yield comparable to fadR expression, indicating that FadR enhanced fatty acid production globally by tuning the expression levels of many genes to optimal levels.

KW - 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase

KW - Acetyl-CoA Carboxylase

KW - Acetyltransferases

KW - Bacterial Proteins

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Fatty Acid Synthetase Complex, Type II

KW - Fatty Acids

KW - Gene Expression Regulation, Enzymologic

KW - Genetic Enhancement

KW - Regulatory Elements, Transcriptional

KW - Repressor Proteins

U2 - 10.1016/j.ymben.2012.08.009

DO - 10.1016/j.ymben.2012.08.009

M3 - Journal article

C2 - 23026122

VL - 14

SP - 653

EP - 660

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

IS - 6

ER -

ID: 68161222