Directly from the source: endogenous preparations of molecular machines

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Directly from the source : endogenous preparations of molecular machines. / Mesa, Pablo; Deniaud, Aurélien; Montoya, Guillermo; Schaffitzel, Christiane.

In: Current Opinion in Structural Biology, Vol. 23, No. 3, 06.2013, p. 319-25.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Mesa, P, Deniaud, A, Montoya, G & Schaffitzel, C 2013, 'Directly from the source: endogenous preparations of molecular machines', Current Opinion in Structural Biology, vol. 23, no. 3, pp. 319-25. https://doi.org/10.1016/j.sbi.2013.01.005

APA

Mesa, P., Deniaud, A., Montoya, G., & Schaffitzel, C. (2013). Directly from the source: endogenous preparations of molecular machines. Current Opinion in Structural Biology, 23(3), 319-25. https://doi.org/10.1016/j.sbi.2013.01.005

Vancouver

Mesa P, Deniaud A, Montoya G, Schaffitzel C. Directly from the source: endogenous preparations of molecular machines. Current Opinion in Structural Biology. 2013 Jun;23(3):319-25. https://doi.org/10.1016/j.sbi.2013.01.005

Author

Mesa, Pablo ; Deniaud, Aurélien ; Montoya, Guillermo ; Schaffitzel, Christiane. / Directly from the source : endogenous preparations of molecular machines. In: Current Opinion in Structural Biology. 2013 ; Vol. 23, No. 3. pp. 319-25.

Bibtex

@article{023f19160e0a47fe899f559df8d693a1,
title = "Directly from the source: endogenous preparations of molecular machines",
abstract = "Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.",
keywords = "Analytic Sample Preparation Methods, Animals, Bacteria/chemistry, Crystallization, DNA, Recombinant/genetics, Databases, Protein, Humans, Multiprotein Complexes/chemistry, Protein Conformation, Recombinant Proteins/biosynthesis, Yeasts/chemistry",
author = "Pablo Mesa and Aur{\'e}lien Deniaud and Guillermo Montoya and Christiane Schaffitzel",
note = "Copyright {\textcopyright} 2013. Published by Elsevier Ltd.",
year = "2013",
month = jun,
doi = "10.1016/j.sbi.2013.01.005",
language = "English",
volume = "23",
pages = "319--25",
journal = "Current Opinion in Structural Biology",
issn = "0959-440X",
publisher = "Elsevier Ltd. * Current Opinion Journals",
number = "3",

}

RIS

TY - JOUR

T1 - Directly from the source

T2 - endogenous preparations of molecular machines

AU - Mesa, Pablo

AU - Deniaud, Aurélien

AU - Montoya, Guillermo

AU - Schaffitzel, Christiane

N1 - Copyright © 2013. Published by Elsevier Ltd.

PY - 2013/6

Y1 - 2013/6

N2 - Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.

AB - Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.

KW - Analytic Sample Preparation Methods

KW - Animals

KW - Bacteria/chemistry

KW - Crystallization

KW - DNA, Recombinant/genetics

KW - Databases, Protein

KW - Humans

KW - Multiprotein Complexes/chemistry

KW - Protein Conformation

KW - Recombinant Proteins/biosynthesis

KW - Yeasts/chemistry

U2 - 10.1016/j.sbi.2013.01.005

DO - 10.1016/j.sbi.2013.01.005

M3 - Review

C2 - 23395510

VL - 23

SP - 319

EP - 325

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

SN - 0959-440X

IS - 3

ER -

ID: 246724916