CRISPR-Cas12a: functional overview and applications

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CRISPR-Cas12a : functional overview and applications. / Paul, Bijoya; Montoya, Guillermo.

In: Biomedical Journal, Vol. 43, No. 1, 2020, p. 8-17.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Paul, B & Montoya, G 2020, 'CRISPR-Cas12a: functional overview and applications', Biomedical Journal, vol. 43, no. 1, pp. 8-17. https://doi.org/10.1016/j.bj.2019.10.005

APA

Paul, B., & Montoya, G. (2020). CRISPR-Cas12a: functional overview and applications. Biomedical Journal, 43(1), 8-17. https://doi.org/10.1016/j.bj.2019.10.005

Vancouver

Paul B, Montoya G. CRISPR-Cas12a: functional overview and applications. Biomedical Journal. 2020;43(1):8-17. https://doi.org/10.1016/j.bj.2019.10.005

Author

Paul, Bijoya ; Montoya, Guillermo. / CRISPR-Cas12a : functional overview and applications. In: Biomedical Journal. 2020 ; Vol. 43, No. 1. pp. 8-17.

Bibtex

@article{2e89cf163f1c4188a2726fd84b9e76b1,
title = "CRISPR-Cas12a: functional overview and applications",
abstract = "Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution. Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape. In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in genome editing and biotechnology applications.",
keywords = "CRISPR-Cas12a, crRNA biogenesis, Endonuclease recycling, Genome editing, Indiscriminate ssDNAse, RNA guided endonucleases",
author = "Bijoya Paul and Guillermo Montoya",
year = "2020",
doi = "10.1016/j.bj.2019.10.005",
language = "English",
volume = "43",
pages = "8--17",
journal = "Chang Gung Medical Journal",
issn = "2319-4170",
publisher = "Medknow Publications and Media Pvt. Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - CRISPR-Cas12a

T2 - functional overview and applications

AU - Paul, Bijoya

AU - Montoya, Guillermo

PY - 2020

Y1 - 2020

N2 - Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution. Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape. In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in genome editing and biotechnology applications.

AB - Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution. Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape. In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in genome editing and biotechnology applications.

KW - CRISPR-Cas12a

KW - crRNA biogenesis

KW - Endonuclease recycling

KW - Genome editing

KW - Indiscriminate ssDNAse

KW - RNA guided endonucleases

U2 - 10.1016/j.bj.2019.10.005

DO - 10.1016/j.bj.2019.10.005

M3 - Review

C2 - 32200959

AN - SCOPUS:85078910313

VL - 43

SP - 8

EP - 17

JO - Chang Gung Medical Journal

JF - Chang Gung Medical Journal

SN - 2319-4170

IS - 1

ER -

ID: 237842978