Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication. / Reverón-Gómez, Nazaret; González-Aguilera, Cristina; Stewart-Morgan, Kathleen R.; Petryk, Nataliya; Flury, Valentin; Graziano, Simona; Johansen, Jens Vilstrup; Jakobsen, Janus Schou; Alabert, Constance; Groth, Anja.

In: Molecular Cell, Vol. 72, No. 2, 2018, p. 239-249.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Reverón-Gómez, N, González-Aguilera, C, Stewart-Morgan, KR, Petryk, N, Flury, V, Graziano, S, Johansen, JV, Jakobsen, JS, Alabert, C & Groth, A 2018, 'Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication', Molecular Cell, vol. 72, no. 2, pp. 239-249. https://doi.org/10.1016/j.molcel.2018.08.010

APA

Reverón-Gómez, N., González-Aguilera, C., Stewart-Morgan, K. R., Petryk, N., Flury, V., Graziano, S., Johansen, J. V., Jakobsen, J. S., Alabert, C., & Groth, A. (2018). Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication. Molecular Cell, 72(2), 239-249. https://doi.org/10.1016/j.molcel.2018.08.010

Vancouver

Reverón-Gómez N, González-Aguilera C, Stewart-Morgan KR, Petryk N, Flury V, Graziano S et al. Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication. Molecular Cell. 2018;72(2):239-249. https://doi.org/10.1016/j.molcel.2018.08.010

Author

Reverón-Gómez, Nazaret ; González-Aguilera, Cristina ; Stewart-Morgan, Kathleen R. ; Petryk, Nataliya ; Flury, Valentin ; Graziano, Simona ; Johansen, Jens Vilstrup ; Jakobsen, Janus Schou ; Alabert, Constance ; Groth, Anja. / Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication. In: Molecular Cell. 2018 ; Vol. 72, No. 2. pp. 239-249.

Bibtex

@article{71ed63a17e304d71bb428aabf8a2ff7f,
title = "Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication",
abstract = "Chromatin organization is disrupted genome-wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naive histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is faithfully copied during DNA replication or the epigenome is perturbed. Here we develop chromatin occupancy after replication (ChOR-seq) to determine histone PTM occupancy immediately after DNA replication and across the cell cycle. We show that H3K4me3, H3K36me3, H3K79me3, and H3K27me3 positional information is reproduced with high accuracy on newly synthesized DNA through histone recycling. Quantitative ChOR-seq reveals that de novo methylation to restore H3K4me3 and H3K27me3 levels occurs across the cell cycle with mark- and locus-specific kinetics. Collectively, this demonstrates that accurate parental histone recycling preserves positional information and allows PTM transmission to daughter cells while modification of new histones gives rise to complex epigenome fluctuations across the cell cycle that could underlie cell-to-cell heterogeneity.",
author = "Nazaret Rever{\'o}n-G{\'o}mez and Cristina Gonz{\'a}lez-Aguilera and Stewart-Morgan, {Kathleen R.} and Nataliya Petryk and Valentin Flury and Simona Graziano and Johansen, {Jens Vilstrup} and Jakobsen, {Janus Schou} and Constance Alabert and Anja Groth",
note = "Copyright {\textcopyright} 2018 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2018",
doi = "10.1016/j.molcel.2018.08.010",
language = "English",
volume = "72",
pages = "239--249",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication

AU - Reverón-Gómez, Nazaret

AU - González-Aguilera, Cristina

AU - Stewart-Morgan, Kathleen R.

AU - Petryk, Nataliya

AU - Flury, Valentin

AU - Graziano, Simona

AU - Johansen, Jens Vilstrup

AU - Jakobsen, Janus Schou

AU - Alabert, Constance

AU - Groth, Anja

N1 - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2018

Y1 - 2018

N2 - Chromatin organization is disrupted genome-wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naive histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is faithfully copied during DNA replication or the epigenome is perturbed. Here we develop chromatin occupancy after replication (ChOR-seq) to determine histone PTM occupancy immediately after DNA replication and across the cell cycle. We show that H3K4me3, H3K36me3, H3K79me3, and H3K27me3 positional information is reproduced with high accuracy on newly synthesized DNA through histone recycling. Quantitative ChOR-seq reveals that de novo methylation to restore H3K4me3 and H3K27me3 levels occurs across the cell cycle with mark- and locus-specific kinetics. Collectively, this demonstrates that accurate parental histone recycling preserves positional information and allows PTM transmission to daughter cells while modification of new histones gives rise to complex epigenome fluctuations across the cell cycle that could underlie cell-to-cell heterogeneity.

AB - Chromatin organization is disrupted genome-wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naive histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is faithfully copied during DNA replication or the epigenome is perturbed. Here we develop chromatin occupancy after replication (ChOR-seq) to determine histone PTM occupancy immediately after DNA replication and across the cell cycle. We show that H3K4me3, H3K36me3, H3K79me3, and H3K27me3 positional information is reproduced with high accuracy on newly synthesized DNA through histone recycling. Quantitative ChOR-seq reveals that de novo methylation to restore H3K4me3 and H3K27me3 levels occurs across the cell cycle with mark- and locus-specific kinetics. Collectively, this demonstrates that accurate parental histone recycling preserves positional information and allows PTM transmission to daughter cells while modification of new histones gives rise to complex epigenome fluctuations across the cell cycle that could underlie cell-to-cell heterogeneity.

U2 - 10.1016/j.molcel.2018.08.010

DO - 10.1016/j.molcel.2018.08.010

M3 - Journal article

C2 - 30146316

VL - 72

SP - 239

EP - 249

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 2

ER -

ID: 202025442