A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy. / Bruhn, Sören; Fang, Yu; Barrenäs, Fredrik; Gustafsson, Mika; Zhang, Huan; Konstantinell, Aelita; Krönke, Andrea; Sönnichsen, Birte; Bresnick, Anne; Dulyaninova, Natalya; Wang, Hui; Zhao, Yelin; Klingelhöfer, Jörg; Ambartsumian, Noona; Beck, Mette K; Nestor, Colm; Bona, Elsa; Xiang, Zou; Benson, Mikael.

In: Science Translational Medicine, Vol. 6, No. 218, 08.01.2014, p. 218ra4.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bruhn, S, Fang, Y, Barrenäs, F, Gustafsson, M, Zhang, H, Konstantinell, A, Krönke, A, Sönnichsen, B, Bresnick, A, Dulyaninova, N, Wang, H, Zhao, Y, Klingelhöfer, J, Ambartsumian, N, Beck, MK, Nestor, C, Bona, E, Xiang, Z & Benson, M 2014, 'A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy', Science Translational Medicine, vol. 6, no. 218, pp. 218ra4. https://doi.org/10.1126/scitranslmed.3007410

APA

Bruhn, S., Fang, Y., Barrenäs, F., Gustafsson, M., Zhang, H., Konstantinell, A., Krönke, A., Sönnichsen, B., Bresnick, A., Dulyaninova, N., Wang, H., Zhao, Y., Klingelhöfer, J., Ambartsumian, N., Beck, M. K., Nestor, C., Bona, E., Xiang, Z., & Benson, M. (2014). A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy. Science Translational Medicine, 6(218), 218ra4. https://doi.org/10.1126/scitranslmed.3007410

Vancouver

Bruhn S, Fang Y, Barrenäs F, Gustafsson M, Zhang H, Konstantinell A et al. A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy. Science Translational Medicine. 2014 Jan 8;6(218):218ra4. https://doi.org/10.1126/scitranslmed.3007410

Author

Bruhn, Sören ; Fang, Yu ; Barrenäs, Fredrik ; Gustafsson, Mika ; Zhang, Huan ; Konstantinell, Aelita ; Krönke, Andrea ; Sönnichsen, Birte ; Bresnick, Anne ; Dulyaninova, Natalya ; Wang, Hui ; Zhao, Yelin ; Klingelhöfer, Jörg ; Ambartsumian, Noona ; Beck, Mette K ; Nestor, Colm ; Bona, Elsa ; Xiang, Zou ; Benson, Mikael. / A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy. In: Science Translational Medicine. 2014 ; Vol. 6, No. 218. pp. 218ra4.

Bibtex

@article{48148878deb64a2abe1ebb6c2f7d2ead,
title = "A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy",
abstract = "The identification of diagnostic markers and therapeutic candidate genes in common diseases is complicated by the involvement of thousands of genes. We hypothesized that genes co-regulated with a key gene in allergy, IL13, would form a module that could help to identify candidate genes. We identified a T helper 2 (TH2) cell module by small interfering RNA-mediated knockdown of 25 putative IL13-regulating transcription factors followed by expression profiling. The module contained candidate genes whose diagnostic potential was supported by clinical studies. Functional studies of human TH2 cells as well as mouse models of allergy showed that deletion of one of the genes, S100A4, resulted in decreased signs of allergy including TH2 cell activation, humoral immunity, and infiltration of effector cells. Specifically, dendritic cells required S100A4 for activating T cells. Treatment with an anti-S100A4 antibody resulted in decreased signs of allergy in the mouse model as well as in allergen-challenged T cells from allergic patients. This strategy, which may be generally applicable to complex diseases, identified and validated an important diagnostic and therapeutic candidate gene in allergy.",
author = "S{\"o}ren Bruhn and Yu Fang and Fredrik Barren{\"a}s and Mika Gustafsson and Huan Zhang and Aelita Konstantinell and Andrea Kr{\"o}nke and Birte S{\"o}nnichsen and Anne Bresnick and Natalya Dulyaninova and Hui Wang and Yelin Zhao and J{\"o}rg Klingelh{\"o}fer and Noona Ambartsumian and Beck, {Mette K} and Colm Nestor and Elsa Bona and Zou Xiang and Mikael Benson",
year = "2014",
month = jan,
day = "8",
doi = "10.1126/scitranslmed.3007410",
language = "English",
volume = "6",
pages = "218ra4",
journal = "Science Translational Medicine",
issn = "1946-6234",
publisher = "american association for the advancement of science",
number = "218",

}

RIS

TY - JOUR

T1 - A generally applicable translational strategy identifies S100A4 as a candidate gene in allergy

AU - Bruhn, Sören

AU - Fang, Yu

AU - Barrenäs, Fredrik

AU - Gustafsson, Mika

AU - Zhang, Huan

AU - Konstantinell, Aelita

AU - Krönke, Andrea

AU - Sönnichsen, Birte

AU - Bresnick, Anne

AU - Dulyaninova, Natalya

AU - Wang, Hui

AU - Zhao, Yelin

AU - Klingelhöfer, Jörg

AU - Ambartsumian, Noona

AU - Beck, Mette K

AU - Nestor, Colm

AU - Bona, Elsa

AU - Xiang, Zou

AU - Benson, Mikael

PY - 2014/1/8

Y1 - 2014/1/8

N2 - The identification of diagnostic markers and therapeutic candidate genes in common diseases is complicated by the involvement of thousands of genes. We hypothesized that genes co-regulated with a key gene in allergy, IL13, would form a module that could help to identify candidate genes. We identified a T helper 2 (TH2) cell module by small interfering RNA-mediated knockdown of 25 putative IL13-regulating transcription factors followed by expression profiling. The module contained candidate genes whose diagnostic potential was supported by clinical studies. Functional studies of human TH2 cells as well as mouse models of allergy showed that deletion of one of the genes, S100A4, resulted in decreased signs of allergy including TH2 cell activation, humoral immunity, and infiltration of effector cells. Specifically, dendritic cells required S100A4 for activating T cells. Treatment with an anti-S100A4 antibody resulted in decreased signs of allergy in the mouse model as well as in allergen-challenged T cells from allergic patients. This strategy, which may be generally applicable to complex diseases, identified and validated an important diagnostic and therapeutic candidate gene in allergy.

AB - The identification of diagnostic markers and therapeutic candidate genes in common diseases is complicated by the involvement of thousands of genes. We hypothesized that genes co-regulated with a key gene in allergy, IL13, would form a module that could help to identify candidate genes. We identified a T helper 2 (TH2) cell module by small interfering RNA-mediated knockdown of 25 putative IL13-regulating transcription factors followed by expression profiling. The module contained candidate genes whose diagnostic potential was supported by clinical studies. Functional studies of human TH2 cells as well as mouse models of allergy showed that deletion of one of the genes, S100A4, resulted in decreased signs of allergy including TH2 cell activation, humoral immunity, and infiltration of effector cells. Specifically, dendritic cells required S100A4 for activating T cells. Treatment with an anti-S100A4 antibody resulted in decreased signs of allergy in the mouse model as well as in allergen-challenged T cells from allergic patients. This strategy, which may be generally applicable to complex diseases, identified and validated an important diagnostic and therapeutic candidate gene in allergy.

U2 - 10.1126/scitranslmed.3007410

DO - 10.1126/scitranslmed.3007410

M3 - Journal article

C2 - 24401939

VL - 6

SP - 218ra4

JO - Science Translational Medicine

JF - Science Translational Medicine

SN - 1946-6234

IS - 218

ER -

ID: 120079337