The ubiquitin ligase RFWD3 is required for translesion DNA synthesis

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Lesions on DNA uncouple DNA synthesis from the replisome, generating stretches of unreplicated single-stranded DNA (ssDNA) behind the replication fork. These ssDNA gaps need to be filled in to complete DNA duplication. Gap-filling synthesis involves either translesion DNA synthesis (TLS) or template switching (TS). Controlling these processes, ubiquitylated PCNA recruits many proteins that dictate pathway choice, but the enzymes regulating PCNA ubiquitylation in vertebrates remain poorly defined. Here we report that the E3 ubiquitin ligase RFWD3 promotes ubiquitylation of proteins on ssDNA. The absence of RFWD3 leads to a profound defect in recruitment of key repair and signaling factors to damaged chromatin. As a result, PCNA ubiquitylation is inhibited without RFWD3, and TLS across different DNA lesions is drastically impaired. We propose that RFWD3 is an essential coordinator of the response to ssDNA gaps, where it promotes ubiquitylation to drive recruitment of effectors of PCNA ubiquitylation and DNA damage bypass.

Original languageEnglish
JournalMolecular Cell
Issue number3
Pages (from-to)442-458.e9
Number of pages27
Publication statusPublished - 2021

    Research areas

  • Animals, Cell Line, Tumor, Chromatin/genetics, DNA Breaks, Single-Stranded, DNA Repair, DNA Replication, DNA-Directed DNA Polymerase/metabolism, Female, Humans, Proliferating Cell Nuclear Antigen/genetics, Substrate Specificity, Ubiquitin-Protein Ligases/genetics, Ubiquitination, Xenopus laevis

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