Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Standard

Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry. / Larsen, Sara C; Leutert, Mario; Bilan, Vera; Martello, Rita; Jungmichel, Stephanie; Young, Clifford; Hottiger, Michael O; Nielsen, Michael L.

Poly(ADP-Ribose) Polymerase. ed. / Alexei V. Tulin. Vol. 1608 Humana Press, 2017. p. 149-162 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Harvard

Larsen, SC, Leutert, M, Bilan, V, Martello, R, Jungmichel, S, Young, C, Hottiger, MO & Nielsen, ML 2017, Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry. in AV Tulin (ed.), Poly(ADP-Ribose) Polymerase. vol. 1608, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 149-162. https://doi.org/10.1007/978-1-4939-6993-7_11

APA

Larsen, S. C., Leutert, M., Bilan, V., Martello, R., Jungmichel, S., Young, C., ... Nielsen, M. L. (2017). Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry. In A. V. Tulin (Ed.), Poly(ADP-Ribose) Polymerase (Vol. 1608, pp. 149-162). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-4939-6993-7_11

Vancouver

Larsen SC, Leutert M, Bilan V, Martello R, Jungmichel S, Young C et al. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry. In Tulin AV, editor, Poly(ADP-Ribose) Polymerase. Vol. 1608. Humana Press. 2017. p. 149-162. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-6993-7_11

Author

Larsen, Sara C ; Leutert, Mario ; Bilan, Vera ; Martello, Rita ; Jungmichel, Stephanie ; Young, Clifford ; Hottiger, Michael O ; Nielsen, Michael L. / Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry. Poly(ADP-Ribose) Polymerase. editor / Alexei V. Tulin. Vol. 1608 Humana Press, 2017. pp. 149-162 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{99cb09d9bb4443058a8c1fa5a13a66fd,
title = "Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry",
abstract = "ADP-ribosylation is a posttranslational modification (PTM) that affects a variety of cellular processes. In recent years, mass spectrometry (MS)-based proteomics has become a valuable tool for studying ADP-ribosylation. However, studying this PTM in vivo in an unbiased and sensitive manner has remained a difficult challenge. Here, we describe a detailed protocol for unbiased analysis of ADP-ribosylated proteins and their ADP-ribose acceptor sites under physiological conditions. The method relies on the enrichment of mono-ADP-ribosylated peptides using the macrodomain Af1521 in combination with liquid chromatography-high-resolution tandem MS (LC-MS/MS). The 5-day protocol explains the step-by-step enrichment and identification of ADP-ribosylated peptides from cell culture stage all the way through to data processing using the MaxQuant software suite.",
keywords = "Journal Article",
author = "Larsen, {Sara C} and Mario Leutert and Vera Bilan and Rita Martello and Stephanie Jungmichel and Clifford Young and Hottiger, {Michael O} and Nielsen, {Michael L}",
year = "2017",
doi = "10.1007/978-1-4939-6993-7_11",
language = "English",
isbn = "978-1-4939-6992-0",
volume = "1608",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "149--162",
editor = "Tulin, {Alexei V.}",
booktitle = "Poly(ADP-Ribose) Polymerase",
address = "United States",

}

RIS

TY - CHAP

T1 - Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography-Tandem Mass Spectrometry

AU - Larsen, Sara C

AU - Leutert, Mario

AU - Bilan, Vera

AU - Martello, Rita

AU - Jungmichel, Stephanie

AU - Young, Clifford

AU - Hottiger, Michael O

AU - Nielsen, Michael L

PY - 2017

Y1 - 2017

N2 - ADP-ribosylation is a posttranslational modification (PTM) that affects a variety of cellular processes. In recent years, mass spectrometry (MS)-based proteomics has become a valuable tool for studying ADP-ribosylation. However, studying this PTM in vivo in an unbiased and sensitive manner has remained a difficult challenge. Here, we describe a detailed protocol for unbiased analysis of ADP-ribosylated proteins and their ADP-ribose acceptor sites under physiological conditions. The method relies on the enrichment of mono-ADP-ribosylated peptides using the macrodomain Af1521 in combination with liquid chromatography-high-resolution tandem MS (LC-MS/MS). The 5-day protocol explains the step-by-step enrichment and identification of ADP-ribosylated peptides from cell culture stage all the way through to data processing using the MaxQuant software suite.

AB - ADP-ribosylation is a posttranslational modification (PTM) that affects a variety of cellular processes. In recent years, mass spectrometry (MS)-based proteomics has become a valuable tool for studying ADP-ribosylation. However, studying this PTM in vivo in an unbiased and sensitive manner has remained a difficult challenge. Here, we describe a detailed protocol for unbiased analysis of ADP-ribosylated proteins and their ADP-ribose acceptor sites under physiological conditions. The method relies on the enrichment of mono-ADP-ribosylated peptides using the macrodomain Af1521 in combination with liquid chromatography-high-resolution tandem MS (LC-MS/MS). The 5-day protocol explains the step-by-step enrichment and identification of ADP-ribosylated peptides from cell culture stage all the way through to data processing using the MaxQuant software suite.

KW - Journal Article

U2 - 10.1007/978-1-4939-6993-7_11

DO - 10.1007/978-1-4939-6993-7_11

M3 - Book chapter

C2 - 28695509

SN - 978-1-4939-6992-0

VL - 1608

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 149

EP - 162

BT - Poly(ADP-Ribose) Polymerase

A2 - Tulin, Alexei V.

PB - Humana Press

ER -

ID: 187623120