MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome

Research output: Contribution to journalJournal articleResearchpeer-review

  • Elena Chiavacci
  • Romina Daurizio
  • Elena Guzzolino
  • Russo, Francesco
  • Mario Baumgart
  • Marco Groth
  • Laura Mariani
  • Mara Donofrio
  • Ivan Arisi
  • Marco Pellegrini
  • Alessandro Cellerino
  • Federico Cremisi
  • Letizia Pitto

Holt-Oram Syndrome (HOS) is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene, a transcription factor capable of regulating hundreds of cardiac-specific genes through complex transcriptional networks. Here we show that, in zebrafish, modulation of a single miRNA is sufficient to rescue the morphogenetic defects generated by HOS. The analysis of miRNA-seq profiling revealed a decreased expression of miR-19a in Tbx5-depleted zebrafish embryos compared to the wild type. We revealed that the transcription of the miR-17-92 cluster, which harbors miR-19a, is induced by Tbx5 and that a defined dosage of miR-19a is essential for the correct development of the heart. Importantly, we highlighted that miR-19a replacement is able to rescue cardiac and pectoral fin defects and to increase the viability of HOS zebrafish embryos. We further observed that miR-19a replacement shifts the global gene expression profile of HOS-like zebrafish embryos towards the wild type condition, confirming the ability of miR-19a to rescue the Tbx5 phenotype. In conclusion our data demonstrate the importance of Tbx5/miR-19a regulatory circuit in heart development and provide a proof of principle that morphogenetic defects associated with HOS can be rescued by transient miRNA modulation.

Original languageEnglish
Article number18240
JournalScientific Reports
Volume5
ISSN2045-2322
DOIs
Publication statusPublished - 14 Dec 2015

ID: 209065633