High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. / Lang, Benjamin; Blot, Nicolas; Bouffartigues, Emeline; Buckle, Malcolm; Geertz, Marcel; Gualerzi, Claudio O.; Mavathur, Ramesh; Muskhelishvili, Georgi; Pon, Cynthia L.; Rimsky, Sylvie; Stella, Stefano; Babu, M. Madan; Travers, Andrew.

In: Nucleic Acids Research, Vol. 35, No. 18, 01.09.2007, p. 6330-6337.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Lang, B, Blot, N, Bouffartigues, E, Buckle, M, Geertz, M, Gualerzi, CO, Mavathur, R, Muskhelishvili, G, Pon, CL, Rimsky, S, Stella, S, Babu, MM & Travers, A 2007, 'High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes', Nucleic Acids Research, vol. 35, no. 18, pp. 6330-6337. https://doi.org/10.1093/nar/gkm712

APA

Lang, B., Blot, N., Bouffartigues, E., Buckle, M., Geertz, M., Gualerzi, C. O., ... Travers, A. (2007). High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. Nucleic Acids Research, 35(18), 6330-6337. https://doi.org/10.1093/nar/gkm712

Vancouver

Lang B, Blot N, Bouffartigues E, Buckle M, Geertz M, Gualerzi CO et al. High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. Nucleic Acids Research. 2007 Sep 1;35(18):6330-6337. https://doi.org/10.1093/nar/gkm712

Author

Lang, Benjamin ; Blot, Nicolas ; Bouffartigues, Emeline ; Buckle, Malcolm ; Geertz, Marcel ; Gualerzi, Claudio O. ; Mavathur, Ramesh ; Muskhelishvili, Georgi ; Pon, Cynthia L. ; Rimsky, Sylvie ; Stella, Stefano ; Babu, M. Madan ; Travers, Andrew. / High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. In: Nucleic Acids Research. 2007 ; Vol. 35, No. 18. pp. 6330-6337.

Bibtex

@article{5d06d8de6d2e4354919740a8faab2bdb,
title = "High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes",
abstract = "The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that there is a strong tendency for the H-NS binding sites to be clustered, both within operons and in genes contained in the pathogenicity-associated islands. In accordance with previously published findings, we show that these motifs occur in AT-rich regions of DNA. On the basis of these observations, we propose that H-NS silences extensive regions of the bacterial chromosome by binding first to nucleating high-affinity sites and then spreading along AT-rich DNA. This spreading would be reinforced by the frequent occurrence of the motif in such regions. Our findings suggest that such an organization enables the silencing of extensive regions of the genetic material, thereby providing a coherent framework that unifies studies on the H-NS protein and a concrete molecular basis for the genetic control of H-NS transcriptional silencing.",
author = "Benjamin Lang and Nicolas Blot and Emeline Bouffartigues and Malcolm Buckle and Marcel Geertz and Gualerzi, {Claudio O.} and Ramesh Mavathur and Georgi Muskhelishvili and Pon, {Cynthia L.} and Sylvie Rimsky and Stefano Stella and Babu, {M. Madan} and Andrew Travers",
year = "2007",
month = "9",
day = "1",
doi = "10.1093/nar/gkm712",
language = "English",
volume = "35",
pages = "6330--6337",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "18",

}

RIS

TY - JOUR

T1 - High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes

AU - Lang, Benjamin

AU - Blot, Nicolas

AU - Bouffartigues, Emeline

AU - Buckle, Malcolm

AU - Geertz, Marcel

AU - Gualerzi, Claudio O.

AU - Mavathur, Ramesh

AU - Muskhelishvili, Georgi

AU - Pon, Cynthia L.

AU - Rimsky, Sylvie

AU - Stella, Stefano

AU - Babu, M. Madan

AU - Travers, Andrew

PY - 2007/9/1

Y1 - 2007/9/1

N2 - The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that there is a strong tendency for the H-NS binding sites to be clustered, both within operons and in genes contained in the pathogenicity-associated islands. In accordance with previously published findings, we show that these motifs occur in AT-rich regions of DNA. On the basis of these observations, we propose that H-NS silences extensive regions of the bacterial chromosome by binding first to nucleating high-affinity sites and then spreading along AT-rich DNA. This spreading would be reinforced by the frequent occurrence of the motif in such regions. Our findings suggest that such an organization enables the silencing of extensive regions of the genetic material, thereby providing a coherent framework that unifies studies on the H-NS protein and a concrete molecular basis for the genetic control of H-NS transcriptional silencing.

AB - The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that there is a strong tendency for the H-NS binding sites to be clustered, both within operons and in genes contained in the pathogenicity-associated islands. In accordance with previously published findings, we show that these motifs occur in AT-rich regions of DNA. On the basis of these observations, we propose that H-NS silences extensive regions of the bacterial chromosome by binding first to nucleating high-affinity sites and then spreading along AT-rich DNA. This spreading would be reinforced by the frequent occurrence of the motif in such regions. Our findings suggest that such an organization enables the silencing of extensive regions of the genetic material, thereby providing a coherent framework that unifies studies on the H-NS protein and a concrete molecular basis for the genetic control of H-NS transcriptional silencing.

UR - http://www.scopus.com/inward/record.url?scp=35549009343&partnerID=8YFLogxK

U2 - 10.1093/nar/gkm712

DO - 10.1093/nar/gkm712

M3 - Journal article

C2 - 17881364

AN - SCOPUS:35549009343

VL - 35

SP - 6330

EP - 6337

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 18

ER -

ID: 202333300