Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells

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To test the hypothesis that whole-body heat acclimation (HA) would increase peripheral blood mononuclear cells' (PBMC) tolerance to heat shock (HS) and/or alter the release of cytokines (IL-1β, IL-6, IL-10 and TNF-α) to bacterial lipopolysaccharide (LPS), we heat acclimated nine subjects by exercising them for 100 min in a hot environment for 10 days. The subjects' PBMC were separated and cultured on days 1 and 10 of HA pre- and post-exercise. Pre-exercise PBMC were allocated to three treatments: control (PRE, 37°C), HS (42.5°C for 2 h), or LPS (1 ng mL(-1) for 24 h). Post-exercise samples were incubated at 37°C. PBMC lactate dehydrogenase release increased (p <0.05) (p after but different hs it not was> 0.05) between days 1 and 10 (0.100 ± 0.012 and 0.102 ± 0.16 abs., respectively). LPS treatment induced an increased (p <0.05) (p alter but cytokines did ha not of release response this> 0.05). Pre-exercise intracellular heat shock protein 72 (Hsp72) was higher (p < 0.05) on day 10 compared to day 1 of HA (13 ± 5 and 8 ± 5 ng mL(-1), respectively). HS treatment caused a greater increase (p < 0.05) in Hsp72 than the exercise sessions on HA days 1 and 10. In addition, after HA, the Hsp72 response to HS was reduced (day 1, 129 ± 46; day 10, 80 ± 32 ng mL(-1), p < 0.05). In conclusion, HA increases PBMC Hsp72 but it does not reduce cellular damage to HS or alter cytokine response to LPS. We speculate that the stress applied during HA is not sufficient to modify the PBMC response.

Original languageEnglish
JournalEuropean Journal of Applied Physiology
Volume111
Issue number8
Pages (from-to)1609-18
Number of pages10
ISSN1439-6319
DOIs
Publication statusPublished - Aug 2011

    Research areas

  • Acclimatization/physiology, Adult, Body Temperature Regulation/physiology, Cells, Cultured, Cytokines/metabolism, Environment, Controlled, Exercise/physiology, Exercise Test, Female, Heat-Shock Response/physiology, Hot Temperature, Humans, Leukocytes, Mononuclear/metabolism, Male, Young Adult

ID: 202296658