Ctk1 function is necessary for full translation initiation activity in Saccharomyces cerevisiae

Research output: Contribution to journalJournal articleResearchpeer-review

  • Britta Coordes
  • Katharina M Brünger
  • Kaspar Burger
  • Boumediene Soufi
  • Juliane Horenk
  • Dirk Eick
  • Olsen, Jesper Velgaard
  • Katja Sträßer

Translation is a fundamental and highly regulated cellular process. Previously, we reported that the kinase and transcription elongation factor Ctk1 increases fidelity during translation elongation in Saccharomyces cerevisiae. Here, we show that loss of Ctk1 function also affects the initiation step of translation. Translation active extracts from Ctk1 depleted cells show impaired translation activity of capped, but not CrPV IRES containing mRNA reporters. Furthermore, the formation of 80S initiation complexes is decreased, which is probably due to reduced subunit joining. In addition, we determined the changes in the phosphorylation pattern of a ribosome enriched fraction after depletion of Ctk1. Thus, we provide a catalogue of phosphoproteomic changes dependent on Ctk1. Taken together, our data suggest a stimulatory function of Ctk1 in 80S formation during translation initiation.

Original languageEnglish
JournalEukaryotic Cell (Online Edition)
Issue number1
Pages (from-to)86
Number of pages95
Publication statusPublished - Jan 2015

ID: 128736574