Characterization of gadolinium complexes for SAD phasing in macromolecular crystallography: application to CbpF

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Seven Gd complexes were used in the preparation of heavy-atom derivatives for solving the structure of choline-binding protein F (CbpF), a 36 kDa surface protein from Streptococcus pneumoniae, by the SAD method. CbpF was used as a model system to analyse the phasing capability of each of the derivatives. Three different aspects have been systematically characterized: the efficacy of cocrystallization versus soaking in the binding of the different Gd complexes, their mode of interaction and a comparative study of SAD phasing using synchrotron radiation and using a rotating-anode generator. This study reveals the striking potential of these complexes for SAD phasing using a laboratory source and further reinforces their relevance for high-throughput macromolecular crystallography.

Original languageEnglish
JournalActa crystallographica. Section D, Biological crystallography
Volume65
Issue numberPt 8
Pages (from-to)823-31
Number of pages9
ISSN2059-7983
DOIs
Publication statusPublished - Aug 2009

    Research areas

  • Bacterial Proteins/chemistry, Carrier Proteins/chemistry, Choline/metabolism, Crystallization, Gadolinium/chemistry, Macromolecular Substances/chemistry, Protein Binding, Protein Conformation, Streptococcus pneumoniae, Sulfur/chemistry, Synchrotrons, X-Ray Diffraction

ID: 203019559