Methods for the identification of PTEN-targeting microRNAs

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The identification of PTEN -targeting microRNAs usually starts from an in silico bioinformatic prediction and then requires a careful experimental validation that exploits both heterologous and endogenous systems. Here we describe the methods used to carry on these analyses and experiments, examining pitfalls and alternatives for each step. Moreover, we give an overview of the latest high-throughput microRNA target identification techniques which offer a more comprehensive view of the microRNAs that can bind a fundamental tumor suppressor such as PTEN.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages28
PublisherHumana Press
Publication date1 Jan 2016
Pages111-138
DOIs
Publication statusPublished - 1 Jan 2016
SeriesMethods in Molecular Biology
Volume1388
ISSN1064-3745

    Research areas

  • 3′ untranslated region, MicroRNA responsive element, MicroRNAs, Posttranscriptional regulation, PTEN

ID: 209064981