Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity

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Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. / Fenton, Thomas M.; Jørgensen, Peter B.; Niss, Kristoffer; Rubin, Samuel J.S.; Mörbe, Urs M.; Riis, Lene B.; Da Silva, Clément; Plumb, Adam; Vandamme, Julien; Jakobsen, Henrik L.; Brunak, Søren; Habtezion, Aida; Nielsen, Ole H.; Johansson-Lindbom, Bengt; Agace, William W.

In: Immunity, Vol. 52, No. 3, 2020, p. 557-570, e1-e6.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Fenton, TM, Jørgensen, PB, Niss, K, Rubin, SJS, Mörbe, UM, Riis, LB, Da Silva, C, Plumb, A, Vandamme, J, Jakobsen, HL, Brunak, S, Habtezion, A, Nielsen, OH, Johansson-Lindbom, B & Agace, WW 2020, 'Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity', Immunity, vol. 52, no. 3, pp. 557-570, e1-e6. https://doi.org/10.1016/j.immuni.2020.02.001

APA

Fenton, T. M., Jørgensen, P. B., Niss, K., Rubin, S. J. S., Mörbe, U. M., Riis, L. B., ... Agace, W. W. (2020). Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. Immunity, 52(3), 557-570, e1-e6. https://doi.org/10.1016/j.immuni.2020.02.001

Vancouver

Fenton TM, Jørgensen PB, Niss K, Rubin SJS, Mörbe UM, Riis LB et al. Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. Immunity. 2020;52(3):557-570, e1-e6. https://doi.org/10.1016/j.immuni.2020.02.001

Author

Fenton, Thomas M. ; Jørgensen, Peter B. ; Niss, Kristoffer ; Rubin, Samuel J.S. ; Mörbe, Urs M. ; Riis, Lene B. ; Da Silva, Clément ; Plumb, Adam ; Vandamme, Julien ; Jakobsen, Henrik L. ; Brunak, Søren ; Habtezion, Aida ; Nielsen, Ole H. ; Johansson-Lindbom, Bengt ; Agace, William W. / Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. In: Immunity. 2020 ; Vol. 52, No. 3. pp. 557-570, e1-e6.

Bibtex

@article{304df76a94114949bbbd7585634fd308,
title = "Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity",
abstract = "The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.",
keywords = "colon, human intestinal regional mucosal follicles, IgA, iIeum, ILF, PP",
author = "Fenton, {Thomas M.} and J{\o}rgensen, {Peter B.} and Kristoffer Niss and Rubin, {Samuel J.S.} and M{\"o}rbe, {Urs M.} and Riis, {Lene B.} and {Da Silva}, Cl{\'e}ment and Adam Plumb and Julien Vandamme and Jakobsen, {Henrik L.} and S{\o}ren Brunak and Aida Habtezion and Nielsen, {Ole H.} and Bengt Johansson-Lindbom and Agace, {William W.}",
year = "2020",
doi = "10.1016/j.immuni.2020.02.001",
language = "English",
volume = "52",
pages = "557--570, e1--e6",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity

AU - Fenton, Thomas M.

AU - Jørgensen, Peter B.

AU - Niss, Kristoffer

AU - Rubin, Samuel J.S.

AU - Mörbe, Urs M.

AU - Riis, Lene B.

AU - Da Silva, Clément

AU - Plumb, Adam

AU - Vandamme, Julien

AU - Jakobsen, Henrik L.

AU - Brunak, Søren

AU - Habtezion, Aida

AU - Nielsen, Ole H.

AU - Johansson-Lindbom, Bengt

AU - Agace, William W.

PY - 2020

Y1 - 2020

N2 - The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.

AB - The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.

KW - colon

KW - human intestinal regional mucosal follicles

KW - IgA

KW - iIeum

KW - ILF

KW - PP

U2 - 10.1016/j.immuni.2020.02.001

DO - 10.1016/j.immuni.2020.02.001

M3 - Journal article

C2 - 32160523

AN - SCOPUS:85081279962

VL - 52

SP - 557-570, e1-e6

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 3

ER -

ID: 237999668