Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

Research output: Contribution to journalJournal articleResearchpeer-review

  • Irina Borodina
  • Kanchana R Kildegaard
  • Niels B Jensen
  • Thomas H Blicher
  • Jérôme Maury
  • Svetlana Sherstyk
  • Konstantin Schneider
  • Pedro Lamosa
  • Markus J Herrgård
  • Inger Rosenstand
  • Fredrik Oberg
  • Jochen Forster
  • Jens Nielsen

Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the β-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of β-alanine and its subsequent conversion into 3HP using a novel β-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7±0.3gL(-1) with a 0.14±0.0C-molC-mol(-1) yield on glucose in 80h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.

Original languageEnglish
JournalMetabolic Engineering
Pages (from-to)57-64
Number of pages8
Publication statusPublished - Jan 2015

ID: 128737752