Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system. / Ejaz, Rooshanie N.; Taylor, Nicholas M.I.; Steiner-Rebrova, Eva M.

In: STAR Protocols, Vol. 5, No. 2, 103014, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ejaz, RN, Taylor, NMI & Steiner-Rebrova, EM 2024, 'Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system', STAR Protocols, vol. 5, no. 2, 103014. https://doi.org/10.1016/j.xpro.2024.103014

APA

Ejaz, R. N., Taylor, N. M. I., & Steiner-Rebrova, E. M. (2024). Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system. STAR Protocols, 5(2), [103014]. https://doi.org/10.1016/j.xpro.2024.103014

Vancouver

Ejaz RN, Taylor NMI, Steiner-Rebrova EM. Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system. STAR Protocols. 2024;5(2). 103014. https://doi.org/10.1016/j.xpro.2024.103014

Author

Ejaz, Rooshanie N. ; Taylor, Nicholas M.I. ; Steiner-Rebrova, Eva M. / Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system. In: STAR Protocols. 2024 ; Vol. 5, No. 2.

Bibtex

@article{78e2266a0e4747a48997ae4a3f9fab3b,
title = "Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system",
abstract = "Genomic manipulation of Yersinia ruckeri, a pathogen of salmonid fish species, is essential for understanding bacterial physiology and virulence. Here, we present a protocol for genomic recombineering in Y. ruckeri, a species reluctant to standard genomic engineering, using CRISPR Cas12a coupled with the λ Red system. We describe steps for identifying protospacer guides, preparing repair template plasmids, and electroporating Yersinia cells with Cpf1 and protospacer plasmids with homologous arms. We then detail procedures for genome editing and plasmid curing.",
keywords = "Biotechnology and bioengineering, CRISPR, Genomics",
author = "Ejaz, {Rooshanie N.} and Taylor, {Nicholas M.I.} and Steiner-Rebrova, {Eva M.}",
note = "Publisher Copyright: {\textcopyright} 2024 The Authors",
year = "2024",
doi = "10.1016/j.xpro.2024.103014",
language = "English",
volume = "5",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system

AU - Ejaz, Rooshanie N.

AU - Taylor, Nicholas M.I.

AU - Steiner-Rebrova, Eva M.

N1 - Publisher Copyright: © 2024 The Authors

PY - 2024

Y1 - 2024

N2 - Genomic manipulation of Yersinia ruckeri, a pathogen of salmonid fish species, is essential for understanding bacterial physiology and virulence. Here, we present a protocol for genomic recombineering in Y. ruckeri, a species reluctant to standard genomic engineering, using CRISPR Cas12a coupled with the λ Red system. We describe steps for identifying protospacer guides, preparing repair template plasmids, and electroporating Yersinia cells with Cpf1 and protospacer plasmids with homologous arms. We then detail procedures for genome editing and plasmid curing.

AB - Genomic manipulation of Yersinia ruckeri, a pathogen of salmonid fish species, is essential for understanding bacterial physiology and virulence. Here, we present a protocol for genomic recombineering in Y. ruckeri, a species reluctant to standard genomic engineering, using CRISPR Cas12a coupled with the λ Red system. We describe steps for identifying protospacer guides, preparing repair template plasmids, and electroporating Yersinia cells with Cpf1 and protospacer plasmids with homologous arms. We then detail procedures for genome editing and plasmid curing.

KW - Biotechnology and bioengineering

KW - CRISPR

KW - Genomics

U2 - 10.1016/j.xpro.2024.103014

DO - 10.1016/j.xpro.2024.103014

M3 - Journal article

C2 - 38615317

AN - SCOPUS:85190088394

VL - 5

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 103014

ER -

ID: 390184224